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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) <t>NSE</t> concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available <t>ELISA</t> kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, <t>NSE:</t> <t>Neuron-specific</t> enolase, S100b: S100 calcium-binding protein B.
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Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) NSE concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available ELISA kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, NSE: Neuron-specific enolase, S100b: S100 calcium-binding protein B.

Journal: Biomedicines

Article Title: Methamphetamine Use in People Living with HIV: Clinical, Neurocognitive, and Blood Biomarker Profiles

doi: 10.3390/biomedicines14020443

Figure Lengend Snippet: Systemic concentrations of biomarkers related to Inflammation and Neuronal Damage. ( A ) sCD14 concentrations; ( B ) NfL concentrations; ( C ) NSE concentrations and ( D ) S100b. All the biomarkers were measured in serum samples (blood-derived) with commercially available ELISA kits. Values represented in the graphs show the median and interquartile ranges (IQR 1–3). Kruskal–Wallis’s test with Dunn’s post hoc test p -value < 0.05 was considered statistically significant. MAHIV: Methamphetamine users who live with HIV, PLWH: People Living with HIV, HIV: Human Immunodeficiency virus, sCD14: soluble CD14, NfL: Neurofilament light chain, NSE: Neuron-specific enolase, S100b: S100 calcium-binding protein B.

Article Snippet: Circulating biomarkers were quantified by enzyme-linked immunosorbent assay (ELISA) using commercial kits: Human soluble CD14 (sCD14) ELISA kit (catalog ID: DC140, R&D Systems, Minneapolis, MN, USA); Human neuron-specific enolase (NSE) ELISA kit, Abclonal (catalog ID: RK01966-96T, ABclonal Science, Wuhan, China); Human S100 calcium-binding protein B (S100B) ELISA kit, (catalog ID: EEL045, Invitrogen, Carlsbad, CA, USA)); and Human neurofilament light chain (NEFL) ELISA kit (catalog ID: E-EL- H26203 , Elabscience, Houston, TX, USA).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Virus, Binding Assay